This paper describes a method that produces amplified signals from single RNA molecules, using Hybridization Chain Reaction and probes in which the HCR initiator sequence is conditionally sequestered. The binding of probes to a target unmasks the initiator and generates an amplified signal. These probes enable detection of sparsely expressed mRNAs and are so specific that related mutant and wild-type mRNAs, differing from each other by as little as a single-nucleotide variation, yield distinct in situ signals in two distinguishable colors. This technology will aid in studies of differential expression from alleles and will be useful for the identification of mRNAs harboring somatic mutations in cancer tissue biopsies.
April 12, 2019.
LaTasha Fraser successfully defends her Ph.D. dissertation, "Exploring the Role of Chromatin Decondensation at a Gene Locus in Stochastic Gene Expression".