©2018 by Sanjay Tyagi Lab. Proudly created with Wix.com

Protocols

Flow cytometric analysis of RNA in cells in suspension | Detection of cytokine mRNAs by flow-cytometry | Analysis of T cell activation by using RNA targets | Multiplex flow-cytometric analysis of RNA and proteins

Labeling and purification of single-molecule FISH probes | Hybridization with tissue culture cells, yeast, C. elegans embryos, Drosophila melanogaster wing imaginal discs | Fade resistant mounting medium | Image acquisition and analysis

Important tip:  Prepare the 0.1M sodium bicarbonate solution (pH 8.3) fresh right before you couple succinimidyl ester derivatives of dyes to probes with amino groups.  The pH will be correct without addition of any base.  The pH will drift upon storage.

Important tip:  Prepare the 0.1M sodium bicarbonate solution (pH 8.3) fresh right before you couple succinimidyl ester derivatives of dyes to probes with amino groups.  The pH will be correct without addition of any base.  The pH will drift upon storage.

Synthesis, purification and characterization of molecular beacons | Measurements of signal-to-background ratios

Important tip:  Use fresh sodium bicarbonate solutions (see above).